EZ DNA Methylation-Gold Kit
The EZ DNA Methylation-Gold Kit is a refinement of our popular EZ DNA Methylation kits. These products consolidate DNA denaturation and bisulfite conversion processes into one step, leading to a much faster bisulfite conversion. This is accomplished using temperature denaturation instead of chemical denaturation which is used in the original EZ DNA Methylation Kit. Also, the EZ DNA Methylation-Gold Kits have been streamlined for high yield recovery of DNA following bisulfite treatment. Recovered bisulfite-converted DNA is ideal for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc.
The EZ DNA Methylation-Gold Kit:
- Complete bisulfite conversion of GC-rich DNA in less than 3 hours.
- A coupled heat denaturation/conversion reaction step steamlines the conversion of non-methylated cytosines into uracil.
- Desulfonation and recovery of bisulfite-treated DNA with a spin column.
- Recovered DNA is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc.