ZymoPURE II Plasmid Isolation kits
ZymoPURE II Plasmid Isolation kits provide the fastest and simplest method available to efficiently isolate transfection quality plasmid DNA from E. coli. The ZymoPURE Plasmid Midiprep kit features a spin column-based method for the purification of up to 400 µg of high-quality plasmid DNA in less than 18 minutes (video). The eluted plasmid DNA is ready for immediate use, avoiding the need for subsequent precipitation steps.
ZymoPURE II technology uses a modified alkaline lysis method and features novel binding chemistry that yields highly concentrated plasmid DNA (up to 3 µg/µl) directly from a spin column. The wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, protein, and RNA resulting in plasmid DNA suitable for sensitive applications including transfection, plasmid-mediated gene silencing, cloning, and sequencing.
As an added convenience, the ZymoPURE II Plasmid Midiprep contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization. Syringe filters are included for rapid clearing of the lysate and the unique spin-column design allows the binding step to be performed using a vacuum or table top centrifuge.